Exp Dermatol. 2015 Mar 23. doi: 10.1111/exd.12700. [Epub ahead of print]
Mapping the expression of epithelial hair follicle stem and progenitor cell-related transcription factors LHX2 and SOX9 in the human hair follicle.
Abstract
In the murine hair follicle (HF) the transcription factors LHX2 and SOX9 are involved with epithelial hair follicle stem cell (eHFSC) self-renewal and the maintenance of eHFSC niche characteristics. However, the exact expression patterns of LHX2 and SOX9 in human HF are unclear. We have quantitatively mapped the localisation of known human eHFSC markers keratin 15 (K15) and keratin 19 (K19) in the ORS of male occipital scalp anagen HFs, and related this to the localisation of LHX2 and SOX9 protein expression. As expected, K15+ and K19+ cells represented two distinct progenitor cell populations in the bulge and in the proximal bulb ORS (pbORS). Interestingly, cell fluorescence for K19 was significantly stronger within the pbORS versus the bulge, and vice versa for K15, describing a hitherto unrecognised differential expression pattern. LHX2 and SOX9 expressing cells were distributed throughout the ORS, including the bulge, but were not restricted to it. SOX9 expression was most prominent in the ORS immediately below the human bulge whereas LHX2+ cells were similarly distributed between the sub-bulge and pbORS, i.e. compartments not enriched with quiescent eHFSCs. During catagen development, the intensity of LHX2 and SOX9 protein expression increased in the proximal HF epithelium. Double-immunostaining showed that SOX9+ cells in human anagen HF epithelium generally do not express K15, K19 or Lhx2. This data suggests that LHX2 and SOX9 may highlight distinct epithelial progenitor cell populations, in addition to K15+ or K19+ cells, that could play an important role in the maintenance of human HF epithelium. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
No comments:
Post a Comment